GrapevineDiseaseV1, Main, Exploration, bibRecord, 000376

Lessons from a Phenotyping Center Revealed by the Genome-Guided Mapping of Powdery Mildew Resistance Loci.

Identifieur interne : 000376 ( Main/Exploration ); précédent : 000375; suivant : 000377

Lessons from a Phenotyping Center Revealed by the Genome-Guided Mapping of Powdery Mildew Resistance Loci.

Auteurs : Lance Cadle-Davidson [États-Unis] ; David Gadoury [États-Unis] ; Jonathan Fresnedo-Ramírez [États-Unis] ; Shanshan Yang [États-Unis] ; Paola Barba [États-Unis] ; Qi Sun [États-Unis] ; Elizabeth M. Demmings [États-Unis] ; Robert Seem [États-Unis] ; Michelle Schaub [États-Unis] ; Anna Nowogrodzki [États-Unis] ; Hema Kasinathan [États-Unis] ; Craig Ledbetter [États-Unis] ; Bruce I. Reisch [États-Unis]

Source :

Phytopathology [ 0031-949X ] ; 2016.

RBID : pubmed:27135675

Descripteurs français

KwdFr :
- Ascomycota (physiologie), Cartographie chromosomique (méthodes), Feuilles de plante (génétique), Feuilles de plante (immunologie), Feuilles de plante (microbiologie), Génome végétal (génétique), Génotype (MeSH), Locus de caractère quantitatif (génétique), Locus génétiques (génétique), Maladies des plantes (immunologie), Maladies des plantes (microbiologie), Phénotype (MeSH), Résistance à la maladie (génétique), Sélection (MeSH), Techniques de génotypage (MeSH), Vitis (génétique), Vitis (immunologie), Vitis (microbiologie).
MESH :
- génétique : Feuilles de plante, Génome végétal, Locus de caractère quantitatif, Locus génétiques, Résistance à la maladie, Vitis.
- immunologie : Feuilles de plante, Maladies des plantes, Vitis.
- microbiologie : Feuilles de plante, Maladies des plantes, Vitis.
- méthodes : Cartographie chromosomique.
- physiologie : Ascomycota.
- Génotype, Phénotype, Sélection, Techniques de génotypage.

English descriptors

KwdEn :
- Ascomycota (physiology), Breeding (MeSH), Chromosome Mapping (methods), Disease Resistance (genetics), Genetic Loci (genetics), Genome, Plant (genetics), Genotype (MeSH), Genotyping Techniques (MeSH), Phenotype (MeSH), Plant Diseases (immunology), Plant Diseases (microbiology), Plant Leaves (genetics), Plant Leaves (immunology), Plant Leaves (microbiology), Quantitative Trait Loci (genetics), Vitis (genetics), Vitis (immunology), Vitis (microbiology).
MESH :
- genetics : Disease Resistance, Genetic Loci, Genome, Plant, Plant Leaves, Quantitative Trait Loci, Vitis.
- immunology : Plant Diseases, Plant Leaves, Vitis.
- methods : Chromosome Mapping.
- microbiology : Plant Diseases, Plant Leaves, Vitis.
- physiology : Ascomycota.
- Breeding, Genotype, Genotyping Techniques, Phenotype.

Abstract

The genomics era brought unprecedented opportunities for genetic analysis of host resistance, but it came with the challenge that accurate and reproducible phenotypes are needed so that genomic results appropriately reflect biology. Phenotyping host resistance by natural infection in the field can produce variable results due to the uncontrolled environment, uneven distribution and genetics of the pathogen, and developmentally regulated resistance among other factors. To address these challenges, we developed highly controlled, standardized methodologies for phenotyping powdery mildew resistance in the context of a phenotyping center, receiving samples of up to 140 grapevine progeny per F₁ family. We applied these methodologies to F₁ families segregating for REN1- or REN2-mediated resistance and validated that some but not all bioassays identified the REN1 or REN2 locus. A point-intercept method (hyphal transects) to quantify colony density objectively at 8 or 9 days postinoculation proved to be the phenotypic response most reproducibly predicted by these resistance loci. Quantitative trait locus (QTL) mapping with genotyping-by-sequencing maps defined the REN1 and REN2 loci at relatively high resolution. In the reference PN40024 genome under each QTL, nucleotide-binding site-leucine-rich repeat candidate resistance genes were identified-one gene for REN1 and two genes for REN2. The methods described here for centralized resistance phenotyping and high-resolution genetic mapping can inform strategies for breeding resistance to powdery mildews and other pathogens on diverse, highly heterozygous hosts.

DOI: 10.1094/PHYTO-02-16-0080-FI
PubMed: 27135675

Affiliations:

Links toward previous steps (curation, corpus...)

to stream Main, to step Corpus: 000388
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Le document en format XML

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<author><name sortKey="Sun, Qi" sort="Sun, Qi" uniqKey="Sun Q" first="Qi" last="Sun">Qi Sun</name>
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<author><name sortKey="Seem, Robert" sort="Seem, Robert" uniqKey="Seem R" first="Robert" last="Seem">Robert Seem</name>
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</affiliation>
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<author><name sortKey="Schaub, Michelle" sort="Schaub, Michelle" uniqKey="Schaub M" first="Michelle" last="Schaub">Michelle Schaub</name>
<affiliation wicri:level="2"><nlm:affiliation>First and ninth authors: U.S. Department of Agriculture-Agricultural Research Service (USDA-ARS), Grape Genetics Research Unit, Geneva, NY 14456; second, fourth, fifth, seventh, eighth, tenth, eleventh, and thirteenth authors: School of Integrative Plant Science, Cornell University, New York State Agricultural Experiment Station, Geneva 14456; third and sixth authors: Bioinformatics Facility, Cornell University, Ithaca, NY 14853; and twelfth author: USDA-ARS, Crop Disease, Pests and Genetics Unit, Parlier, CA 93648.</nlm:affiliation>
<country xml:lang="fr">États-Unis</country>
<placeName><region type="state">Californie</region>
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<author><name sortKey="Nowogrodzki, Anna" sort="Nowogrodzki, Anna" uniqKey="Nowogrodzki A" first="Anna" last="Nowogrodzki">Anna Nowogrodzki</name>
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</affiliation>
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<author><name sortKey="Kasinathan, Hema" sort="Kasinathan, Hema" uniqKey="Kasinathan H" first="Hema" last="Kasinathan">Hema Kasinathan</name>
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<country xml:lang="fr">États-Unis</country>
<placeName><region type="state">Californie</region>
</placeName>
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</affiliation>
</author>
<author><name sortKey="Ledbetter, Craig" sort="Ledbetter, Craig" uniqKey="Ledbetter C" first="Craig" last="Ledbetter">Craig Ledbetter</name>
<affiliation wicri:level="2"><nlm:affiliation>First and ninth authors: U.S. Department of Agriculture-Agricultural Research Service (USDA-ARS), Grape Genetics Research Unit, Geneva, NY 14456; second, fourth, fifth, seventh, eighth, tenth, eleventh, and thirteenth authors: School of Integrative Plant Science, Cornell University, New York State Agricultural Experiment Station, Geneva 14456; third and sixth authors: Bioinformatics Facility, Cornell University, Ithaca, NY 14853; and twelfth author: USDA-ARS, Crop Disease, Pests and Genetics Unit, Parlier, CA 93648.</nlm:affiliation>
<country xml:lang="fr">États-Unis</country>
<placeName><region type="state">Californie</region>
</placeName>
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</affiliation>
</author>
<author><name sortKey="Reisch, Bruce I" sort="Reisch, Bruce I" uniqKey="Reisch B" first="Bruce I" last="Reisch">Bruce I. Reisch</name>
<affiliation wicri:level="2"><nlm:affiliation>First and ninth authors: U.S. Department of Agriculture-Agricultural Research Service (USDA-ARS), Grape Genetics Research Unit, Geneva, NY 14456; second, fourth, fifth, seventh, eighth, tenth, eleventh, and thirteenth authors: School of Integrative Plant Science, Cornell University, New York State Agricultural Experiment Station, Geneva 14456; third and sixth authors: Bioinformatics Facility, Cornell University, Ithaca, NY 14853; and twelfth author: USDA-ARS, Crop Disease, Pests and Genetics Unit, Parlier, CA 93648.</nlm:affiliation>
<country xml:lang="fr">États-Unis</country>
<placeName><region type="state">Californie</region>
</placeName>
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<sourceDesc><biblStruct><analytic><title xml:lang="en">Lessons from a Phenotyping Center Revealed by the Genome-Guided Mapping of Powdery Mildew Resistance Loci.</title>
<author><name sortKey="Cadle Davidson, Lance" sort="Cadle Davidson, Lance" uniqKey="Cadle Davidson L" first="Lance" last="Cadle-Davidson">Lance Cadle-Davidson</name>
<affiliation wicri:level="2"><nlm:affiliation>First and ninth authors: U.S. Department of Agriculture-Agricultural Research Service (USDA-ARS), Grape Genetics Research Unit, Geneva, NY 14456; second, fourth, fifth, seventh, eighth, tenth, eleventh, and thirteenth authors: School of Integrative Plant Science, Cornell University, New York State Agricultural Experiment Station, Geneva 14456; third and sixth authors: Bioinformatics Facility, Cornell University, Ithaca, NY 14853; and twelfth author: USDA-ARS, Crop Disease, Pests and Genetics Unit, Parlier, CA 93648.</nlm:affiliation>
<country xml:lang="fr">États-Unis</country>
<placeName><region type="state">Californie</region>
</placeName>
<wicri:cityArea>First and ninth authors: U.S. Department of Agriculture-Agricultural Research Service (USDA-ARS), Grape Genetics Research Unit, Geneva, NY 14456; second, fourth, fifth, seventh, eighth, tenth, eleventh, and thirteenth authors: School of Integrative Plant Science, Cornell University, New York State Agricultural Experiment Station, Geneva 14456; third and sixth authors: Bioinformatics Facility, Cornell University, Ithaca, NY 14853; and twelfth author: USDA-ARS, Crop Disease, Pests and Genetics Unit, Parlier</wicri:cityArea>
</affiliation>
</author>
<author><name sortKey="Gadoury, David" sort="Gadoury, David" uniqKey="Gadoury D" first="David" last="Gadoury">David Gadoury</name>
<affiliation wicri:level="2"><nlm:affiliation>First and ninth authors: U.S. Department of Agriculture-Agricultural Research Service (USDA-ARS), Grape Genetics Research Unit, Geneva, NY 14456; second, fourth, fifth, seventh, eighth, tenth, eleventh, and thirteenth authors: School of Integrative Plant Science, Cornell University, New York State Agricultural Experiment Station, Geneva 14456; third and sixth authors: Bioinformatics Facility, Cornell University, Ithaca, NY 14853; and twelfth author: USDA-ARS, Crop Disease, Pests and Genetics Unit, Parlier, CA 93648.</nlm:affiliation>
<country xml:lang="fr">États-Unis</country>
<placeName><region type="state">Californie</region>
</placeName>
<wicri:cityArea>First and ninth authors: U.S. Department of Agriculture-Agricultural Research Service (USDA-ARS), Grape Genetics Research Unit, Geneva, NY 14456; second, fourth, fifth, seventh, eighth, tenth, eleventh, and thirteenth authors: School of Integrative Plant Science, Cornell University, New York State Agricultural Experiment Station, Geneva 14456; third and sixth authors: Bioinformatics Facility, Cornell University, Ithaca, NY 14853; and twelfth author: USDA-ARS, Crop Disease, Pests and Genetics Unit, Parlier</wicri:cityArea>
</affiliation>
</author>
<author><name sortKey="Fresnedo Ramirez, Jonathan" sort="Fresnedo Ramirez, Jonathan" uniqKey="Fresnedo Ramirez J" first="Jonathan" last="Fresnedo-Ramírez">Jonathan Fresnedo-Ramírez</name>
<affiliation wicri:level="2"><nlm:affiliation>First and ninth authors: U.S. Department of Agriculture-Agricultural Research Service (USDA-ARS), Grape Genetics Research Unit, Geneva, NY 14456; second, fourth, fifth, seventh, eighth, tenth, eleventh, and thirteenth authors: School of Integrative Plant Science, Cornell University, New York State Agricultural Experiment Station, Geneva 14456; third and sixth authors: Bioinformatics Facility, Cornell University, Ithaca, NY 14853; and twelfth author: USDA-ARS, Crop Disease, Pests and Genetics Unit, Parlier, CA 93648.</nlm:affiliation>
<country xml:lang="fr">États-Unis</country>
<placeName><region type="state">Californie</region>
</placeName>
<wicri:cityArea>First and ninth authors: U.S. Department of Agriculture-Agricultural Research Service (USDA-ARS), Grape Genetics Research Unit, Geneva, NY 14456; second, fourth, fifth, seventh, eighth, tenth, eleventh, and thirteenth authors: School of Integrative Plant Science, Cornell University, New York State Agricultural Experiment Station, Geneva 14456; third and sixth authors: Bioinformatics Facility, Cornell University, Ithaca, NY 14853; and twelfth author: USDA-ARS, Crop Disease, Pests and Genetics Unit, Parlier</wicri:cityArea>
</affiliation>
</author>
<author><name sortKey="Yang, Shanshan" sort="Yang, Shanshan" uniqKey="Yang S" first="Shanshan" last="Yang">Shanshan Yang</name>
<affiliation wicri:level="2"><nlm:affiliation>First and ninth authors: U.S. Department of Agriculture-Agricultural Research Service (USDA-ARS), Grape Genetics Research Unit, Geneva, NY 14456; second, fourth, fifth, seventh, eighth, tenth, eleventh, and thirteenth authors: School of Integrative Plant Science, Cornell University, New York State Agricultural Experiment Station, Geneva 14456; third and sixth authors: Bioinformatics Facility, Cornell University, Ithaca, NY 14853; and twelfth author: USDA-ARS, Crop Disease, Pests and Genetics Unit, Parlier, CA 93648.</nlm:affiliation>
<country xml:lang="fr">États-Unis</country>
<placeName><region type="state">Californie</region>
</placeName>
<wicri:cityArea>First and ninth authors: U.S. Department of Agriculture-Agricultural Research Service (USDA-ARS), Grape Genetics Research Unit, Geneva, NY 14456; second, fourth, fifth, seventh, eighth, tenth, eleventh, and thirteenth authors: School of Integrative Plant Science, Cornell University, New York State Agricultural Experiment Station, Geneva 14456; third and sixth authors: Bioinformatics Facility, Cornell University, Ithaca, NY 14853; and twelfth author: USDA-ARS, Crop Disease, Pests and Genetics Unit, Parlier</wicri:cityArea>
</affiliation>
</author>
<author><name sortKey="Barba, Paola" sort="Barba, Paola" uniqKey="Barba P" first="Paola" last="Barba">Paola Barba</name>
<affiliation wicri:level="2"><nlm:affiliation>First and ninth authors: U.S. Department of Agriculture-Agricultural Research Service (USDA-ARS), Grape Genetics Research Unit, Geneva, NY 14456; second, fourth, fifth, seventh, eighth, tenth, eleventh, and thirteenth authors: School of Integrative Plant Science, Cornell University, New York State Agricultural Experiment Station, Geneva 14456; third and sixth authors: Bioinformatics Facility, Cornell University, Ithaca, NY 14853; and twelfth author: USDA-ARS, Crop Disease, Pests and Genetics Unit, Parlier, CA 93648.</nlm:affiliation>
<country xml:lang="fr">États-Unis</country>
<placeName><region type="state">Californie</region>
</placeName>
<wicri:cityArea>First and ninth authors: U.S. Department of Agriculture-Agricultural Research Service (USDA-ARS), Grape Genetics Research Unit, Geneva, NY 14456; second, fourth, fifth, seventh, eighth, tenth, eleventh, and thirteenth authors: School of Integrative Plant Science, Cornell University, New York State Agricultural Experiment Station, Geneva 14456; third and sixth authors: Bioinformatics Facility, Cornell University, Ithaca, NY 14853; and twelfth author: USDA-ARS, Crop Disease, Pests and Genetics Unit, Parlier</wicri:cityArea>
</affiliation>
</author>
<author><name sortKey="Sun, Qi" sort="Sun, Qi" uniqKey="Sun Q" first="Qi" last="Sun">Qi Sun</name>
<affiliation wicri:level="2"><nlm:affiliation>First and ninth authors: U.S. Department of Agriculture-Agricultural Research Service (USDA-ARS), Grape Genetics Research Unit, Geneva, NY 14456; second, fourth, fifth, seventh, eighth, tenth, eleventh, and thirteenth authors: School of Integrative Plant Science, Cornell University, New York State Agricultural Experiment Station, Geneva 14456; third and sixth authors: Bioinformatics Facility, Cornell University, Ithaca, NY 14853; and twelfth author: USDA-ARS, Crop Disease, Pests and Genetics Unit, Parlier, CA 93648.</nlm:affiliation>
<country xml:lang="fr">États-Unis</country>
<placeName><region type="state">Californie</region>
</placeName>
<wicri:cityArea>First and ninth authors: U.S. Department of Agriculture-Agricultural Research Service (USDA-ARS), Grape Genetics Research Unit, Geneva, NY 14456; second, fourth, fifth, seventh, eighth, tenth, eleventh, and thirteenth authors: School of Integrative Plant Science, Cornell University, New York State Agricultural Experiment Station, Geneva 14456; third and sixth authors: Bioinformatics Facility, Cornell University, Ithaca, NY 14853; and twelfth author: USDA-ARS, Crop Disease, Pests and Genetics Unit, Parlier</wicri:cityArea>
</affiliation>
</author>
<author><name sortKey="Demmings, Elizabeth M" sort="Demmings, Elizabeth M" uniqKey="Demmings E" first="Elizabeth M" last="Demmings">Elizabeth M. Demmings</name>
<affiliation wicri:level="2"><nlm:affiliation>First and ninth authors: U.S. Department of Agriculture-Agricultural Research Service (USDA-ARS), Grape Genetics Research Unit, Geneva, NY 14456; second, fourth, fifth, seventh, eighth, tenth, eleventh, and thirteenth authors: School of Integrative Plant Science, Cornell University, New York State Agricultural Experiment Station, Geneva 14456; third and sixth authors: Bioinformatics Facility, Cornell University, Ithaca, NY 14853; and twelfth author: USDA-ARS, Crop Disease, Pests and Genetics Unit, Parlier, CA 93648.</nlm:affiliation>
<country xml:lang="fr">États-Unis</country>
<placeName><region type="state">Californie</region>
</placeName>
<wicri:cityArea>First and ninth authors: U.S. Department of Agriculture-Agricultural Research Service (USDA-ARS), Grape Genetics Research Unit, Geneva, NY 14456; second, fourth, fifth, seventh, eighth, tenth, eleventh, and thirteenth authors: School of Integrative Plant Science, Cornell University, New York State Agricultural Experiment Station, Geneva 14456; third and sixth authors: Bioinformatics Facility, Cornell University, Ithaca, NY 14853; and twelfth author: USDA-ARS, Crop Disease, Pests and Genetics Unit, Parlier</wicri:cityArea>
</affiliation>
</author>
<author><name sortKey="Seem, Robert" sort="Seem, Robert" uniqKey="Seem R" first="Robert" last="Seem">Robert Seem</name>
<affiliation wicri:level="2"><nlm:affiliation>First and ninth authors: U.S. Department of Agriculture-Agricultural Research Service (USDA-ARS), Grape Genetics Research Unit, Geneva, NY 14456; second, fourth, fifth, seventh, eighth, tenth, eleventh, and thirteenth authors: School of Integrative Plant Science, Cornell University, New York State Agricultural Experiment Station, Geneva 14456; third and sixth authors: Bioinformatics Facility, Cornell University, Ithaca, NY 14853; and twelfth author: USDA-ARS, Crop Disease, Pests and Genetics Unit, Parlier, CA 93648.</nlm:affiliation>
<country xml:lang="fr">États-Unis</country>
<placeName><region type="state">Californie</region>
</placeName>
<wicri:cityArea>First and ninth authors: U.S. Department of Agriculture-Agricultural Research Service (USDA-ARS), Grape Genetics Research Unit, Geneva, NY 14456; second, fourth, fifth, seventh, eighth, tenth, eleventh, and thirteenth authors: School of Integrative Plant Science, Cornell University, New York State Agricultural Experiment Station, Geneva 14456; third and sixth authors: Bioinformatics Facility, Cornell University, Ithaca, NY 14853; and twelfth author: USDA-ARS, Crop Disease, Pests and Genetics Unit, Parlier</wicri:cityArea>
</affiliation>
</author>
<author><name sortKey="Schaub, Michelle" sort="Schaub, Michelle" uniqKey="Schaub M" first="Michelle" last="Schaub">Michelle Schaub</name>
<affiliation wicri:level="2"><nlm:affiliation>First and ninth authors: U.S. Department of Agriculture-Agricultural Research Service (USDA-ARS), Grape Genetics Research Unit, Geneva, NY 14456; second, fourth, fifth, seventh, eighth, tenth, eleventh, and thirteenth authors: School of Integrative Plant Science, Cornell University, New York State Agricultural Experiment Station, Geneva 14456; third and sixth authors: Bioinformatics Facility, Cornell University, Ithaca, NY 14853; and twelfth author: USDA-ARS, Crop Disease, Pests and Genetics Unit, Parlier, CA 93648.</nlm:affiliation>
<country xml:lang="fr">États-Unis</country>
<placeName><region type="state">Californie</region>
</placeName>
<wicri:cityArea>First and ninth authors: U.S. Department of Agriculture-Agricultural Research Service (USDA-ARS), Grape Genetics Research Unit, Geneva, NY 14456; second, fourth, fifth, seventh, eighth, tenth, eleventh, and thirteenth authors: School of Integrative Plant Science, Cornell University, New York State Agricultural Experiment Station, Geneva 14456; third and sixth authors: Bioinformatics Facility, Cornell University, Ithaca, NY 14853; and twelfth author: USDA-ARS, Crop Disease, Pests and Genetics Unit, Parlier</wicri:cityArea>
</affiliation>
</author>
<author><name sortKey="Nowogrodzki, Anna" sort="Nowogrodzki, Anna" uniqKey="Nowogrodzki A" first="Anna" last="Nowogrodzki">Anna Nowogrodzki</name>
<affiliation wicri:level="2"><nlm:affiliation>First and ninth authors: U.S. Department of Agriculture-Agricultural Research Service (USDA-ARS), Grape Genetics Research Unit, Geneva, NY 14456; second, fourth, fifth, seventh, eighth, tenth, eleventh, and thirteenth authors: School of Integrative Plant Science, Cornell University, New York State Agricultural Experiment Station, Geneva 14456; third and sixth authors: Bioinformatics Facility, Cornell University, Ithaca, NY 14853; and twelfth author: USDA-ARS, Crop Disease, Pests and Genetics Unit, Parlier, CA 93648.</nlm:affiliation>
<country xml:lang="fr">États-Unis</country>
<placeName><region type="state">Californie</region>
</placeName>
<wicri:cityArea>First and ninth authors: U.S. Department of Agriculture-Agricultural Research Service (USDA-ARS), Grape Genetics Research Unit, Geneva, NY 14456; second, fourth, fifth, seventh, eighth, tenth, eleventh, and thirteenth authors: School of Integrative Plant Science, Cornell University, New York State Agricultural Experiment Station, Geneva 14456; third and sixth authors: Bioinformatics Facility, Cornell University, Ithaca, NY 14853; and twelfth author: USDA-ARS, Crop Disease, Pests and Genetics Unit, Parlier</wicri:cityArea>
</affiliation>
</author>
<author><name sortKey="Kasinathan, Hema" sort="Kasinathan, Hema" uniqKey="Kasinathan H" first="Hema" last="Kasinathan">Hema Kasinathan</name>
<affiliation wicri:level="2"><nlm:affiliation>First and ninth authors: U.S. Department of Agriculture-Agricultural Research Service (USDA-ARS), Grape Genetics Research Unit, Geneva, NY 14456; second, fourth, fifth, seventh, eighth, tenth, eleventh, and thirteenth authors: School of Integrative Plant Science, Cornell University, New York State Agricultural Experiment Station, Geneva 14456; third and sixth authors: Bioinformatics Facility, Cornell University, Ithaca, NY 14853; and twelfth author: USDA-ARS, Crop Disease, Pests and Genetics Unit, Parlier, CA 93648.</nlm:affiliation>
<country xml:lang="fr">États-Unis</country>
<placeName><region type="state">Californie</region>
</placeName>
<wicri:cityArea>First and ninth authors: U.S. Department of Agriculture-Agricultural Research Service (USDA-ARS), Grape Genetics Research Unit, Geneva, NY 14456; second, fourth, fifth, seventh, eighth, tenth, eleventh, and thirteenth authors: School of Integrative Plant Science, Cornell University, New York State Agricultural Experiment Station, Geneva 14456; third and sixth authors: Bioinformatics Facility, Cornell University, Ithaca, NY 14853; and twelfth author: USDA-ARS, Crop Disease, Pests and Genetics Unit, Parlier</wicri:cityArea>
</affiliation>
</author>
<author><name sortKey="Ledbetter, Craig" sort="Ledbetter, Craig" uniqKey="Ledbetter C" first="Craig" last="Ledbetter">Craig Ledbetter</name>
<affiliation wicri:level="2"><nlm:affiliation>First and ninth authors: U.S. Department of Agriculture-Agricultural Research Service (USDA-ARS), Grape Genetics Research Unit, Geneva, NY 14456; second, fourth, fifth, seventh, eighth, tenth, eleventh, and thirteenth authors: School of Integrative Plant Science, Cornell University, New York State Agricultural Experiment Station, Geneva 14456; third and sixth authors: Bioinformatics Facility, Cornell University, Ithaca, NY 14853; and twelfth author: USDA-ARS, Crop Disease, Pests and Genetics Unit, Parlier, CA 93648.</nlm:affiliation>
<country xml:lang="fr">États-Unis</country>
<placeName><region type="state">Californie</region>
</placeName>
<wicri:cityArea>First and ninth authors: U.S. Department of Agriculture-Agricultural Research Service (USDA-ARS), Grape Genetics Research Unit, Geneva, NY 14456; second, fourth, fifth, seventh, eighth, tenth, eleventh, and thirteenth authors: School of Integrative Plant Science, Cornell University, New York State Agricultural Experiment Station, Geneva 14456; third and sixth authors: Bioinformatics Facility, Cornell University, Ithaca, NY 14853; and twelfth author: USDA-ARS, Crop Disease, Pests and Genetics Unit, Parlier</wicri:cityArea>
</affiliation>
</author>
<author><name sortKey="Reisch, Bruce I" sort="Reisch, Bruce I" uniqKey="Reisch B" first="Bruce I" last="Reisch">Bruce I. Reisch</name>
<affiliation wicri:level="2"><nlm:affiliation>First and ninth authors: U.S. Department of Agriculture-Agricultural Research Service (USDA-ARS), Grape Genetics Research Unit, Geneva, NY 14456; second, fourth, fifth, seventh, eighth, tenth, eleventh, and thirteenth authors: School of Integrative Plant Science, Cornell University, New York State Agricultural Experiment Station, Geneva 14456; third and sixth authors: Bioinformatics Facility, Cornell University, Ithaca, NY 14853; and twelfth author: USDA-ARS, Crop Disease, Pests and Genetics Unit, Parlier, CA 93648.</nlm:affiliation>
<country xml:lang="fr">États-Unis</country>
<placeName><region type="state">Californie</region>
</placeName>
<wicri:cityArea>First and ninth authors: U.S. Department of Agriculture-Agricultural Research Service (USDA-ARS), Grape Genetics Research Unit, Geneva, NY 14456; second, fourth, fifth, seventh, eighth, tenth, eleventh, and thirteenth authors: School of Integrative Plant Science, Cornell University, New York State Agricultural Experiment Station, Geneva 14456; third and sixth authors: Bioinformatics Facility, Cornell University, Ithaca, NY 14853; and twelfth author: USDA-ARS, Crop Disease, Pests and Genetics Unit, Parlier</wicri:cityArea>
</affiliation>
</author>
</analytic>
<series><title level="j">Phytopathology</title>
<idno type="ISSN">0031-949X</idno>
<imprint><date when="2016" type="published">2016</date>
</imprint>
</series>
</biblStruct>
</sourceDesc>
</fileDesc>
<profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Ascomycota (physiology)</term>
<term>Breeding (MeSH)</term>
<term>Chromosome Mapping (methods)</term>
<term>Disease Resistance (genetics)</term>
<term>Genetic Loci (genetics)</term>
<term>Genome, Plant (genetics)</term>
<term>Genotype (MeSH)</term>
<term>Genotyping Techniques (MeSH)</term>
<term>Phenotype (MeSH)</term>
<term>Plant Diseases (immunology)</term>
<term>Plant Diseases (microbiology)</term>
<term>Plant Leaves (genetics)</term>
<term>Plant Leaves (immunology)</term>
<term>Plant Leaves (microbiology)</term>
<term>Quantitative Trait Loci (genetics)</term>
<term>Vitis (genetics)</term>
<term>Vitis (immunology)</term>
<term>Vitis (microbiology)</term>
</keywords>
<keywords scheme="KwdFr" xml:lang="fr"><term>Ascomycota (physiologie)</term>
<term>Cartographie chromosomique (méthodes)</term>
<term>Feuilles de plante (génétique)</term>
<term>Feuilles de plante (immunologie)</term>
<term>Feuilles de plante (microbiologie)</term>
<term>Génome végétal (génétique)</term>
<term>Génotype (MeSH)</term>
<term>Locus de caractère quantitatif (génétique)</term>
<term>Locus génétiques (génétique)</term>
<term>Maladies des plantes (immunologie)</term>
<term>Maladies des plantes (microbiologie)</term>
<term>Phénotype (MeSH)</term>
<term>Résistance à la maladie (génétique)</term>
<term>Sélection (MeSH)</term>
<term>Techniques de génotypage (MeSH)</term>
<term>Vitis (génétique)</term>
<term>Vitis (immunologie)</term>
<term>Vitis (microbiologie)</term>
</keywords>
<keywords scheme="MESH" qualifier="genetics" xml:lang="en"><term>Disease Resistance</term>
<term>Genetic Loci</term>
<term>Genome, Plant</term>
<term>Plant Leaves</term>
<term>Quantitative Trait Loci</term>
<term>Vitis</term>
</keywords>
<keywords scheme="MESH" qualifier="génétique" xml:lang="fr"><term>Feuilles de plante</term>
<term>Génome végétal</term>
<term>Locus de caractère quantitatif</term>
<term>Locus génétiques</term>
<term>Résistance à la maladie</term>
<term>Vitis</term>
</keywords>
<keywords scheme="MESH" qualifier="immunologie" xml:lang="fr"><term>Feuilles de plante</term>
<term>Maladies des plantes</term>
<term>Vitis</term>
</keywords>
<keywords scheme="MESH" qualifier="immunology" xml:lang="en"><term>Plant Diseases</term>
<term>Plant Leaves</term>
<term>Vitis</term>
</keywords>
<keywords scheme="MESH" qualifier="methods" xml:lang="en"><term>Chromosome Mapping</term>
</keywords>
<keywords scheme="MESH" qualifier="microbiologie" xml:lang="fr"><term>Feuilles de plante</term>
<term>Maladies des plantes</term>
<term>Vitis</term>
</keywords>
<keywords scheme="MESH" qualifier="microbiology" xml:lang="en"><term>Plant Diseases</term>
<term>Plant Leaves</term>
<term>Vitis</term>
</keywords>
<keywords scheme="MESH" qualifier="méthodes" xml:lang="fr"><term>Cartographie chromosomique</term>
</keywords>
<keywords scheme="MESH" qualifier="physiologie" xml:lang="fr"><term>Ascomycota</term>
</keywords>
<keywords scheme="MESH" qualifier="physiology" xml:lang="en"><term>Ascomycota</term>
</keywords>
<keywords scheme="MESH" xml:lang="en"><term>Breeding</term>
<term>Genotype</term>
<term>Genotyping Techniques</term>
<term>Phenotype</term>
</keywords>
<keywords scheme="MESH" xml:lang="fr"><term>Génotype</term>
<term>Phénotype</term>
<term>Sélection</term>
<term>Techniques de génotypage</term>
</keywords>
</textClass>
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</teiHeader>
<front><div type="abstract" xml:lang="en">The genomics era brought unprecedented opportunities for genetic analysis of host resistance, but it came with the challenge that accurate and reproducible phenotypes are needed so that genomic results appropriately reflect biology. Phenotyping host resistance by natural infection in the field can produce variable results due to the uncontrolled environment, uneven distribution and genetics of the pathogen, and developmentally regulated resistance among other factors. To address these challenges, we developed highly controlled, standardized methodologies for phenotyping powdery mildew resistance in the context of a phenotyping center, receiving samples of up to 140 grapevine progeny per F<sub>1</sub>
 family. We applied these methodologies to F<sub>1</sub>
 families segregating for REN1- or REN2-mediated resistance and validated that some but not all bioassays identified the REN1 or REN2 locus. A point-intercept method (hyphal transects) to quantify colony density objectively at 8 or 9 days postinoculation proved to be the phenotypic response most reproducibly predicted by these resistance loci. Quantitative trait locus (QTL) mapping with genotyping-by-sequencing maps defined the REN1 and REN2 loci at relatively high resolution. In the reference PN40024 genome under each QTL, nucleotide-binding site-leucine-rich repeat candidate resistance genes were identified-one gene for REN1 and two genes for REN2. The methods described here for centralized resistance phenotyping and high-resolution genetic mapping can inform strategies for breeding resistance to powdery mildews and other pathogens on diverse, highly heterozygous hosts.</div>
</front>
</TEI>
<pubmed><MedlineCitation Status="MEDLINE" Owner="NLM"><PMID Version="1">27135675</PMID>
<DateCompleted><Year>2017</Year>
<Month>06</Month>
<Day>29</Day>
</DateCompleted>
<DateRevised><Year>2017</Year>
<Month>06</Month>
<Day>29</Day>
</DateRevised>
<Article PubModel="Print-Electronic"><Journal><ISSN IssnType="Print">0031-949X</ISSN>
<JournalIssue CitedMedium="Print"><Volume>106</Volume>
<Issue>10</Issue>
<PubDate><Year>2016</Year>
<Month>10</Month>
</PubDate>
</JournalIssue>
<Title>Phytopathology</Title>
<ISOAbbreviation>Phytopathology</ISOAbbreviation>
</Journal>
<ArticleTitle>Lessons from a Phenotyping Center Revealed by the Genome-Guided Mapping of Powdery Mildew Resistance Loci.</ArticleTitle>
<Pagination><MedlinePgn>1159-1169</MedlinePgn>
</Pagination>
<Abstract><AbstractText>The genomics era brought unprecedented opportunities for genetic analysis of host resistance, but it came with the challenge that accurate and reproducible phenotypes are needed so that genomic results appropriately reflect biology. Phenotyping host resistance by natural infection in the field can produce variable results due to the uncontrolled environment, uneven distribution and genetics of the pathogen, and developmentally regulated resistance among other factors. To address these challenges, we developed highly controlled, standardized methodologies for phenotyping powdery mildew resistance in the context of a phenotyping center, receiving samples of up to 140 grapevine progeny per F<sub>1</sub>
 family. We applied these methodologies to F<sub>1</sub>
 families segregating for REN1- or REN2-mediated resistance and validated that some but not all bioassays identified the REN1 or REN2 locus. A point-intercept method (hyphal transects) to quantify colony density objectively at 8 or 9 days postinoculation proved to be the phenotypic response most reproducibly predicted by these resistance loci. Quantitative trait locus (QTL) mapping with genotyping-by-sequencing maps defined the REN1 and REN2 loci at relatively high resolution. In the reference PN40024 genome under each QTL, nucleotide-binding site-leucine-rich repeat candidate resistance genes were identified-one gene for REN1 and two genes for REN2. The methods described here for centralized resistance phenotyping and high-resolution genetic mapping can inform strategies for breeding resistance to powdery mildews and other pathogens on diverse, highly heterozygous hosts.</AbstractText>
</Abstract>
<AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>Cadle-Davidson</LastName>
<ForeName>Lance</ForeName>
<Initials>L</Initials>
<AffiliationInfo><Affiliation>First and ninth authors: U.S. Department of Agriculture-Agricultural Research Service (USDA-ARS), Grape Genetics Research Unit, Geneva, NY 14456; second, fourth, fifth, seventh, eighth, tenth, eleventh, and thirteenth authors: School of Integrative Plant Science, Cornell University, New York State Agricultural Experiment Station, Geneva 14456; third and sixth authors: Bioinformatics Facility, Cornell University, Ithaca, NY 14853; and twelfth author: USDA-ARS, Crop Disease, Pests and Genetics Unit, Parlier, CA 93648.</Affiliation>
</AffiliationInfo>
</Author>
<Author ValidYN="Y"><LastName>Gadoury</LastName>
<ForeName>David</ForeName>
<Initials>D</Initials>
<AffiliationInfo><Affiliation>First and ninth authors: U.S. Department of Agriculture-Agricultural Research Service (USDA-ARS), Grape Genetics Research Unit, Geneva, NY 14456; second, fourth, fifth, seventh, eighth, tenth, eleventh, and thirteenth authors: School of Integrative Plant Science, Cornell University, New York State Agricultural Experiment Station, Geneva 14456; third and sixth authors: Bioinformatics Facility, Cornell University, Ithaca, NY 14853; and twelfth author: USDA-ARS, Crop Disease, Pests and Genetics Unit, Parlier, CA 93648.</Affiliation>
</AffiliationInfo>
</Author>
<Author ValidYN="Y"><LastName>Fresnedo-Ramírez</LastName>
<ForeName>Jonathan</ForeName>
<Initials>J</Initials>
<AffiliationInfo><Affiliation>First and ninth authors: U.S. Department of Agriculture-Agricultural Research Service (USDA-ARS), Grape Genetics Research Unit, Geneva, NY 14456; second, fourth, fifth, seventh, eighth, tenth, eleventh, and thirteenth authors: School of Integrative Plant Science, Cornell University, New York State Agricultural Experiment Station, Geneva 14456; third and sixth authors: Bioinformatics Facility, Cornell University, Ithaca, NY 14853; and twelfth author: USDA-ARS, Crop Disease, Pests and Genetics Unit, Parlier, CA 93648.</Affiliation>
</AffiliationInfo>
</Author>
<Author ValidYN="Y"><LastName>Yang</LastName>
<ForeName>Shanshan</ForeName>
<Initials>S</Initials>
<AffiliationInfo><Affiliation>First and ninth authors: U.S. Department of Agriculture-Agricultural Research Service (USDA-ARS), Grape Genetics Research Unit, Geneva, NY 14456; second, fourth, fifth, seventh, eighth, tenth, eleventh, and thirteenth authors: School of Integrative Plant Science, Cornell University, New York State Agricultural Experiment Station, Geneva 14456; third and sixth authors: Bioinformatics Facility, Cornell University, Ithaca, NY 14853; and twelfth author: USDA-ARS, Crop Disease, Pests and Genetics Unit, Parlier, CA 93648.</Affiliation>
</AffiliationInfo>
</Author>
<Author ValidYN="Y"><LastName>Barba</LastName>
<ForeName>Paola</ForeName>
<Initials>P</Initials>
<AffiliationInfo><Affiliation>First and ninth authors: U.S. Department of Agriculture-Agricultural Research Service (USDA-ARS), Grape Genetics Research Unit, Geneva, NY 14456; second, fourth, fifth, seventh, eighth, tenth, eleventh, and thirteenth authors: School of Integrative Plant Science, Cornell University, New York State Agricultural Experiment Station, Geneva 14456; third and sixth authors: Bioinformatics Facility, Cornell University, Ithaca, NY 14853; and twelfth author: USDA-ARS, Crop Disease, Pests and Genetics Unit, Parlier, CA 93648.</Affiliation>
</AffiliationInfo>
</Author>
<Author ValidYN="Y"><LastName>Sun</LastName>
<ForeName>Qi</ForeName>
<Initials>Q</Initials>
<AffiliationInfo><Affiliation>First and ninth authors: U.S. Department of Agriculture-Agricultural Research Service (USDA-ARS), Grape Genetics Research Unit, Geneva, NY 14456; second, fourth, fifth, seventh, eighth, tenth, eleventh, and thirteenth authors: School of Integrative Plant Science, Cornell University, New York State Agricultural Experiment Station, Geneva 14456; third and sixth authors: Bioinformatics Facility, Cornell University, Ithaca, NY 14853; and twelfth author: USDA-ARS, Crop Disease, Pests and Genetics Unit, Parlier, CA 93648.</Affiliation>
</AffiliationInfo>
</Author>
<Author ValidYN="Y"><LastName>Demmings</LastName>
<ForeName>Elizabeth M</ForeName>
<Initials>EM</Initials>
<AffiliationInfo><Affiliation>First and ninth authors: U.S. Department of Agriculture-Agricultural Research Service (USDA-ARS), Grape Genetics Research Unit, Geneva, NY 14456; second, fourth, fifth, seventh, eighth, tenth, eleventh, and thirteenth authors: School of Integrative Plant Science, Cornell University, New York State Agricultural Experiment Station, Geneva 14456; third and sixth authors: Bioinformatics Facility, Cornell University, Ithaca, NY 14853; and twelfth author: USDA-ARS, Crop Disease, Pests and Genetics Unit, Parlier, CA 93648.</Affiliation>
</AffiliationInfo>
</Author>
<Author ValidYN="Y"><LastName>Seem</LastName>
<ForeName>Robert</ForeName>
<Initials>R</Initials>
<AffiliationInfo><Affiliation>First and ninth authors: U.S. Department of Agriculture-Agricultural Research Service (USDA-ARS), Grape Genetics Research Unit, Geneva, NY 14456; second, fourth, fifth, seventh, eighth, tenth, eleventh, and thirteenth authors: School of Integrative Plant Science, Cornell University, New York State Agricultural Experiment Station, Geneva 14456; third and sixth authors: Bioinformatics Facility, Cornell University, Ithaca, NY 14853; and twelfth author: USDA-ARS, Crop Disease, Pests and Genetics Unit, Parlier, CA 93648.</Affiliation>
</AffiliationInfo>
</Author>
<Author ValidYN="Y"><LastName>Schaub</LastName>
<ForeName>Michelle</ForeName>
<Initials>M</Initials>
<AffiliationInfo><Affiliation>First and ninth authors: U.S. Department of Agriculture-Agricultural Research Service (USDA-ARS), Grape Genetics Research Unit, Geneva, NY 14456; second, fourth, fifth, seventh, eighth, tenth, eleventh, and thirteenth authors: School of Integrative Plant Science, Cornell University, New York State Agricultural Experiment Station, Geneva 14456; third and sixth authors: Bioinformatics Facility, Cornell University, Ithaca, NY 14853; and twelfth author: USDA-ARS, Crop Disease, Pests and Genetics Unit, Parlier, CA 93648.</Affiliation>
</AffiliationInfo>
</Author>
<Author ValidYN="Y"><LastName>Nowogrodzki</LastName>
<ForeName>Anna</ForeName>
<Initials>A</Initials>
<AffiliationInfo><Affiliation>First and ninth authors: U.S. Department of Agriculture-Agricultural Research Service (USDA-ARS), Grape Genetics Research Unit, Geneva, NY 14456; second, fourth, fifth, seventh, eighth, tenth, eleventh, and thirteenth authors: School of Integrative Plant Science, Cornell University, New York State Agricultural Experiment Station, Geneva 14456; third and sixth authors: Bioinformatics Facility, Cornell University, Ithaca, NY 14853; and twelfth author: USDA-ARS, Crop Disease, Pests and Genetics Unit, Parlier, CA 93648.</Affiliation>
</AffiliationInfo>
</Author>
<Author ValidYN="Y"><LastName>Kasinathan</LastName>
<ForeName>Hema</ForeName>
<Initials>H</Initials>
<AffiliationInfo><Affiliation>First and ninth authors: U.S. Department of Agriculture-Agricultural Research Service (USDA-ARS), Grape Genetics Research Unit, Geneva, NY 14456; second, fourth, fifth, seventh, eighth, tenth, eleventh, and thirteenth authors: School of Integrative Plant Science, Cornell University, New York State Agricultural Experiment Station, Geneva 14456; third and sixth authors: Bioinformatics Facility, Cornell University, Ithaca, NY 14853; and twelfth author: USDA-ARS, Crop Disease, Pests and Genetics Unit, Parlier, CA 93648.</Affiliation>
</AffiliationInfo>
</Author>
<Author ValidYN="Y"><LastName>Ledbetter</LastName>
<ForeName>Craig</ForeName>
<Initials>C</Initials>
<AffiliationInfo><Affiliation>First and ninth authors: U.S. Department of Agriculture-Agricultural Research Service (USDA-ARS), Grape Genetics Research Unit, Geneva, NY 14456; second, fourth, fifth, seventh, eighth, tenth, eleventh, and thirteenth authors: School of Integrative Plant Science, Cornell University, New York State Agricultural Experiment Station, Geneva 14456; third and sixth authors: Bioinformatics Facility, Cornell University, Ithaca, NY 14853; and twelfth author: USDA-ARS, Crop Disease, Pests and Genetics Unit, Parlier, CA 93648.</Affiliation>
</AffiliationInfo>
</Author>
<Author ValidYN="Y"><LastName>Reisch</LastName>
<ForeName>Bruce I</ForeName>
<Initials>BI</Initials>
<AffiliationInfo><Affiliation>First and ninth authors: U.S. Department of Agriculture-Agricultural Research Service (USDA-ARS), Grape Genetics Research Unit, Geneva, NY 14456; second, fourth, fifth, seventh, eighth, tenth, eleventh, and thirteenth authors: School of Integrative Plant Science, Cornell University, New York State Agricultural Experiment Station, Geneva 14456; third and sixth authors: Bioinformatics Facility, Cornell University, Ithaca, NY 14853; and twelfth author: USDA-ARS, Crop Disease, Pests and Genetics Unit, Parlier, CA 93648.</Affiliation>
</AffiliationInfo>
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</AuthorList>
<Language>eng</Language>
<PublicationTypeList><PublicationType UI="D016428">Journal Article</PublicationType>
</PublicationTypeList>
<ArticleDate DateType="Electronic"><Year>2016</Year>
<Month>07</Month>
<Day>25</Day>
</ArticleDate>
</Article>
<MedlineJournalInfo><Country>United States</Country>
<MedlineTA>Phytopathology</MedlineTA>
<NlmUniqueID>9427222</NlmUniqueID>
<ISSNLinking>0031-949X</ISSNLinking>
</MedlineJournalInfo>
<CitationSubset>IM</CitationSubset>
<MeshHeadingList><MeshHeading><DescriptorName UI="D001203" MajorTopicYN="N">Ascomycota</DescriptorName>
<QualifierName UI="Q000502" MajorTopicYN="Y">physiology</QualifierName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D001947" MajorTopicYN="N">Breeding</DescriptorName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D002874" MajorTopicYN="N">Chromosome Mapping</DescriptorName>
<QualifierName UI="Q000379" MajorTopicYN="Y">methods</QualifierName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D060467" MajorTopicYN="N">Disease Resistance</DescriptorName>
<QualifierName UI="Q000235" MajorTopicYN="Y">genetics</QualifierName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D056426" MajorTopicYN="N">Genetic Loci</DescriptorName>
<QualifierName UI="Q000235" MajorTopicYN="N">genetics</QualifierName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D018745" MajorTopicYN="N">Genome, Plant</DescriptorName>
<QualifierName UI="Q000235" MajorTopicYN="Y">genetics</QualifierName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D005838" MajorTopicYN="N">Genotype</DescriptorName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D060005" MajorTopicYN="N">Genotyping Techniques</DescriptorName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D010641" MajorTopicYN="N">Phenotype</DescriptorName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D010935" MajorTopicYN="N">Plant Diseases</DescriptorName>
<QualifierName UI="Q000276" MajorTopicYN="Y">immunology</QualifierName>
<QualifierName UI="Q000382" MajorTopicYN="N">microbiology</QualifierName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D018515" MajorTopicYN="N">Plant Leaves</DescriptorName>
<QualifierName UI="Q000235" MajorTopicYN="N">genetics</QualifierName>
<QualifierName UI="Q000276" MajorTopicYN="N">immunology</QualifierName>
<QualifierName UI="Q000382" MajorTopicYN="N">microbiology</QualifierName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D040641" MajorTopicYN="N">Quantitative Trait Loci</DescriptorName>
<QualifierName UI="Q000235" MajorTopicYN="Y">genetics</QualifierName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D027843" MajorTopicYN="N">Vitis</DescriptorName>
<QualifierName UI="Q000235" MajorTopicYN="Y">genetics</QualifierName>
<QualifierName UI="Q000276" MajorTopicYN="N">immunology</QualifierName>
<QualifierName UI="Q000382" MajorTopicYN="N">microbiology</QualifierName>
</MeshHeading>
</MeshHeadingList>
</MedlineCitation>
<PubmedData><History><PubMedPubDate PubStatus="entrez"><Year>2016</Year>
<Month>5</Month>
<Day>3</Day>
<Hour>6</Hour>
<Minute>0</Minute>
</PubMedPubDate>
<PubMedPubDate PubStatus="pubmed"><Year>2016</Year>
<Month>5</Month>
<Day>3</Day>
<Hour>6</Hour>
<Minute>0</Minute>
</PubMedPubDate>
<PubMedPubDate PubStatus="medline"><Year>2017</Year>
<Month>7</Month>
<Day>1</Day>
<Hour>6</Hour>
<Minute>0</Minute>
</PubMedPubDate>
</History>
<PublicationStatus>ppublish</PublicationStatus>
<ArticleIdList><ArticleId IdType="pubmed">27135675</ArticleId>
<ArticleId IdType="doi">10.1094/PHYTO-02-16-0080-FI</ArticleId>
</ArticleIdList>
</PubmedData>
</pubmed>
<affiliations><list><country><li>États-Unis</li>
</country>
<region><li>Californie</li>
</region>
</list>
<tree><country name="États-Unis"><region name="Californie"><name sortKey="Cadle Davidson, Lance" sort="Cadle Davidson, Lance" uniqKey="Cadle Davidson L" first="Lance" last="Cadle-Davidson">Lance Cadle-Davidson</name>
</region>
<name sortKey="Barba, Paola" sort="Barba, Paola" uniqKey="Barba P" first="Paola" last="Barba">Paola Barba</name>
<name sortKey="Demmings, Elizabeth M" sort="Demmings, Elizabeth M" uniqKey="Demmings E" first="Elizabeth M" last="Demmings">Elizabeth M. Demmings</name>
<name sortKey="Fresnedo Ramirez, Jonathan" sort="Fresnedo Ramirez, Jonathan" uniqKey="Fresnedo Ramirez J" first="Jonathan" last="Fresnedo-Ramírez">Jonathan Fresnedo-Ramírez</name>
<name sortKey="Gadoury, David" sort="Gadoury, David" uniqKey="Gadoury D" first="David" last="Gadoury">David Gadoury</name>
<name sortKey="Kasinathan, Hema" sort="Kasinathan, Hema" uniqKey="Kasinathan H" first="Hema" last="Kasinathan">Hema Kasinathan</name>
<name sortKey="Ledbetter, Craig" sort="Ledbetter, Craig" uniqKey="Ledbetter C" first="Craig" last="Ledbetter">Craig Ledbetter</name>
<name sortKey="Nowogrodzki, Anna" sort="Nowogrodzki, Anna" uniqKey="Nowogrodzki A" first="Anna" last="Nowogrodzki">Anna Nowogrodzki</name>
<name sortKey="Reisch, Bruce I" sort="Reisch, Bruce I" uniqKey="Reisch B" first="Bruce I" last="Reisch">Bruce I. Reisch</name>
<name sortKey="Schaub, Michelle" sort="Schaub, Michelle" uniqKey="Schaub M" first="Michelle" last="Schaub">Michelle Schaub</name>
<name sortKey="Seem, Robert" sort="Seem, Robert" uniqKey="Seem R" first="Robert" last="Seem">Robert Seem</name>
<name sortKey="Sun, Qi" sort="Sun, Qi" uniqKey="Sun Q" first="Qi" last="Sun">Qi Sun</name>
<name sortKey="Yang, Shanshan" sort="Yang, Shanshan" uniqKey="Yang S" first="Shanshan" last="Yang">Shanshan Yang</name>
</country>
</tree>
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</record>

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	Serveur d'exploration sur les maladies des plantes grimpantes
	Attention, ce site est en cours de développement ! Attention, site généré par des moyens informatiques à partir de corpus bruts. Les informations ne sont donc pas validées.

Serveur d'exploration sur les maladies des plantes grimpantes

Lessons from a Phenotyping Center Revealed by the Genome-Guided Mapping of Powdery Mildew Resistance Loci.

Lessons from a Phenotyping Center Revealed by the Genome-Guided Mapping of Powdery Mildew Resistance Loci.

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